OPINION OF THE COURT ON FURTHER REVIEW
HARVEY, Judge:
A general court-martial composed of officer and enlisted members convicted appellant, contrary to his pleas, of rape, aggravated assault with a dangerous weapon, burglary, and communication of a threat in violation of Articles 120, 128, 129, and 184, Uniform Code of Military Justice, 10 U.S.C. §§ 920, 928, 929, and 934 [hereinafter UCMJ], on 2 November 1996. The convening authority approved the adjudged sentence to a dishonorable discharge, confinement for eight years, forfeiture of all pay and allowances, and reduction to Private El. The forfeitures were deferred on 10 December 1996 until action, and at action were waived for six months with direction that the forfeitures be paid to appellant’s wife and three children. This court set aside the findings of guilty and the sentence because the military judge erroneously failed to grant a challenge for cause against a court member; a rehearing was authorized. United States v. Mason, ARMY 9601811 (Army Ct.Crim.App. 30 Jun. 1999) (unpub.).
Appellant was retried before a general court-martial composed of officer and enlisted members and, contrary to his pleas, on 31 March 2000, was convicted of rape and burglary in violation of Articles 120 and 129, UCMJ. He was sentenced to a dishonorable discharge, confinement for ten years, forfeiture of all pay and allowances, and reduction to Private El. On 18 October 2000, the convening authority approved only so much of the sentence as provides for a dishonorable discharge, confinement for eight years, forfeiture of all pay and allowances, and reduction to Private El.1 The convening authority credited appellant with 992 days of confinement credit.
In this Article 66, UCMJ, 10 U.S.C. § 866, appeal, defense appellate counsel assert, inter alia, that trial counsel’s redirect examination of its deoxyribonucleic acid (DNA) expert improperly shifted the government’s burden of proof to appellant and that the evidence is factually insufficient to support the findings. We disagree.
Facts
At 0529 Specialist (SPC) P, who lived in quarters on Fort Riley with his wife and two children, went to work. His spouse, Mrs. P, stayed in bed with their 18-month-old baby sleeping next to her. A few minutes after SPC P left, Mrs. P heard the front door open. Then she heard someone moving down the hallway towards her bedroom. Mrs. P believed that her husband had returned because he had forgotten his hat. When the person entered her bedroom, she screamed. The person was not her husband. Mrs. P said that the intruder brandished a knife and threatened her son’s life unless she stopped screaming.2 The' intruder then raped Mrs. P. By 0537 the attacker had left Mrs. P’s quarters. At trial and on appeal, the defense did not contest that Mrs. P had been raped.
Mrs. P called her husband at work at about 0537 and told him she had just been raped. She then called the military police. At about 0545, first the military police and then the U.S. Army Criminal Investigation Command (CID) special agents arrived at SPC P’s quarters. Mrs. P described her assailant to CID and at the retrial, as “a [B]lack male, around 5'6" to 5'7" tall, stocky build, around 150 to 160 pounds; he had razor bumps, a big nose____ [and] a slight mustache.” He was dressed in an Army physical training (PT) uniform with a black wool cap. Mrs. P was unable to see her [523]*523attacker’s teeth,3 nor did she describe any other distinguishing features of the rapist. Appellant is a Black male, 5'5" tall, and weighed 172 pounds. At the time of the rape, he had a slight mustache and an intermittent problem with razor bumps.4 Neither SPC P nor Mrs. P knew appellant.
While Mrs. P was being raped, she tried to remove her assailant’s cap to get a better look at his face. He knocked her hand away, covered her eyes, and told her not to look at him. Thereafter, he told her to roll over onto her front, so her face was in her pillow. He continued to engage in sexual intercourse until he ejaculated. Mrs. P’s bedroom was dark; she is near-sighted and was not wearing her glasses during the rape.
Prior to the first trial, Mrs. P was unable to identify her attacker from a physical lineup (which did not include appellant)5 and from a subsequent photo line-up conducted approximately three months after the rape (which included a poor-quality Polaroid photograph of appellant). At the original trial, Mrs. P was asked, “Now, do you know the accused in this case, and he’s sitting between [appellant’s civilian and military defense counsel].” She responded “no.” At the conclusion of the merits testimony during the original trial, the trial judge instructed the members, “[Mrs. P] has been unable to identify any specific person as her attacker in physical or photographic lineups. During her testimony to this court-martial, she was unable to identify the accused as her attacker.”
At the rehearing, Mrs. P testified that in the original trial, before being called as a witness, she saw appellant in the courtroom hallway and recognized him as her attacker. He was wearing his Class A uniform, including his name tag. Mrs. P recognized appellant before she saw his name tag. She told no one except her husband (who was with her at the time), because after recognizing appellant no one asked her to identify him as the perpetrator and she was scared. At the rehearing, Mrs. P also explained that when she testified in the original trial that she did not “know” appellant, she meant she did not know appellant socially and did not work with him. At the rehearing, Mrs. P made an in-court identification of appellant as the man who raped her, without objection from trial defense counsel.
Lieutenant Colonel Garra testified that he overheard Mrs. P tell her husband in a coffee lounge during the first trial that she could not recognize appellant as her attacker. Inmate Brown, who was a neighbor of the P’s, said that in 1997 Mrs. P told him that she did not believe the person in jail was her attacker.
Serology and DNA Evidence
Mrs. P’s vagina was swabbed as part of the rape kit procedure and the swabs and her panties were sent to the USACIL for testing.6 At USACIL, lab personnel found semen on Mrs. P’s panties and on the vaginal swabs. Testing revealed that the rapist had blood-type B, which matched appellant’s blood type. Blood-type B is shared by approximate 19% of the total Black population. Specialist P and three other possible suspects did not have blood-type B.
[524]*524Mr. Auvdel, an expert in DNA analysis who was employed at USACIL, conducted the DNA tests in this case. He used Restriction Fragment Length Polymorphism (RFLP) DNA analysis 7 and Federal Bureau of Investigation (FBI) “match criteria”8 to compare the five chromosome loci in the semen from Mrs. P’s vaginal swabs and panties to five chromosome loci from appellant’s DNA.
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OPINION OF THE COURT ON FURTHER REVIEW
HARVEY, Judge:
A general court-martial composed of officer and enlisted members convicted appellant, contrary to his pleas, of rape, aggravated assault with a dangerous weapon, burglary, and communication of a threat in violation of Articles 120, 128, 129, and 184, Uniform Code of Military Justice, 10 U.S.C. §§ 920, 928, 929, and 934 [hereinafter UCMJ], on 2 November 1996. The convening authority approved the adjudged sentence to a dishonorable discharge, confinement for eight years, forfeiture of all pay and allowances, and reduction to Private El. The forfeitures were deferred on 10 December 1996 until action, and at action were waived for six months with direction that the forfeitures be paid to appellant’s wife and three children. This court set aside the findings of guilty and the sentence because the military judge erroneously failed to grant a challenge for cause against a court member; a rehearing was authorized. United States v. Mason, ARMY 9601811 (Army Ct.Crim.App. 30 Jun. 1999) (unpub.).
Appellant was retried before a general court-martial composed of officer and enlisted members and, contrary to his pleas, on 31 March 2000, was convicted of rape and burglary in violation of Articles 120 and 129, UCMJ. He was sentenced to a dishonorable discharge, confinement for ten years, forfeiture of all pay and allowances, and reduction to Private El. On 18 October 2000, the convening authority approved only so much of the sentence as provides for a dishonorable discharge, confinement for eight years, forfeiture of all pay and allowances, and reduction to Private El.1 The convening authority credited appellant with 992 days of confinement credit.
In this Article 66, UCMJ, 10 U.S.C. § 866, appeal, defense appellate counsel assert, inter alia, that trial counsel’s redirect examination of its deoxyribonucleic acid (DNA) expert improperly shifted the government’s burden of proof to appellant and that the evidence is factually insufficient to support the findings. We disagree.
Facts
At 0529 Specialist (SPC) P, who lived in quarters on Fort Riley with his wife and two children, went to work. His spouse, Mrs. P, stayed in bed with their 18-month-old baby sleeping next to her. A few minutes after SPC P left, Mrs. P heard the front door open. Then she heard someone moving down the hallway towards her bedroom. Mrs. P believed that her husband had returned because he had forgotten his hat. When the person entered her bedroom, she screamed. The person was not her husband. Mrs. P said that the intruder brandished a knife and threatened her son’s life unless she stopped screaming.2 The' intruder then raped Mrs. P. By 0537 the attacker had left Mrs. P’s quarters. At trial and on appeal, the defense did not contest that Mrs. P had been raped.
Mrs. P called her husband at work at about 0537 and told him she had just been raped. She then called the military police. At about 0545, first the military police and then the U.S. Army Criminal Investigation Command (CID) special agents arrived at SPC P’s quarters. Mrs. P described her assailant to CID and at the retrial, as “a [B]lack male, around 5'6" to 5'7" tall, stocky build, around 150 to 160 pounds; he had razor bumps, a big nose____ [and] a slight mustache.” He was dressed in an Army physical training (PT) uniform with a black wool cap. Mrs. P was unable to see her [523]*523attacker’s teeth,3 nor did she describe any other distinguishing features of the rapist. Appellant is a Black male, 5'5" tall, and weighed 172 pounds. At the time of the rape, he had a slight mustache and an intermittent problem with razor bumps.4 Neither SPC P nor Mrs. P knew appellant.
While Mrs. P was being raped, she tried to remove her assailant’s cap to get a better look at his face. He knocked her hand away, covered her eyes, and told her not to look at him. Thereafter, he told her to roll over onto her front, so her face was in her pillow. He continued to engage in sexual intercourse until he ejaculated. Mrs. P’s bedroom was dark; she is near-sighted and was not wearing her glasses during the rape.
Prior to the first trial, Mrs. P was unable to identify her attacker from a physical lineup (which did not include appellant)5 and from a subsequent photo line-up conducted approximately three months after the rape (which included a poor-quality Polaroid photograph of appellant). At the original trial, Mrs. P was asked, “Now, do you know the accused in this case, and he’s sitting between [appellant’s civilian and military defense counsel].” She responded “no.” At the conclusion of the merits testimony during the original trial, the trial judge instructed the members, “[Mrs. P] has been unable to identify any specific person as her attacker in physical or photographic lineups. During her testimony to this court-martial, she was unable to identify the accused as her attacker.”
At the rehearing, Mrs. P testified that in the original trial, before being called as a witness, she saw appellant in the courtroom hallway and recognized him as her attacker. He was wearing his Class A uniform, including his name tag. Mrs. P recognized appellant before she saw his name tag. She told no one except her husband (who was with her at the time), because after recognizing appellant no one asked her to identify him as the perpetrator and she was scared. At the rehearing, Mrs. P also explained that when she testified in the original trial that she did not “know” appellant, she meant she did not know appellant socially and did not work with him. At the rehearing, Mrs. P made an in-court identification of appellant as the man who raped her, without objection from trial defense counsel.
Lieutenant Colonel Garra testified that he overheard Mrs. P tell her husband in a coffee lounge during the first trial that she could not recognize appellant as her attacker. Inmate Brown, who was a neighbor of the P’s, said that in 1997 Mrs. P told him that she did not believe the person in jail was her attacker.
Serology and DNA Evidence
Mrs. P’s vagina was swabbed as part of the rape kit procedure and the swabs and her panties were sent to the USACIL for testing.6 At USACIL, lab personnel found semen on Mrs. P’s panties and on the vaginal swabs. Testing revealed that the rapist had blood-type B, which matched appellant’s blood type. Blood-type B is shared by approximate 19% of the total Black population. Specialist P and three other possible suspects did not have blood-type B.
[524]*524Mr. Auvdel, an expert in DNA analysis who was employed at USACIL, conducted the DNA tests in this case. He used Restriction Fragment Length Polymorphism (RFLP) DNA analysis 7 and Federal Bureau of Investigation (FBI) “match criteria”8 to compare the five chromosome loci in the semen from Mrs. P’s vaginal swabs and panties to five chromosome loci from appellant’s DNA. The National Research Council (NRC)9 guidelines were used to establish the DNA testing procedures at USACIL. Utilizing the FBI database for statistical analysis and applying the “product rule,” Mr. Auvdel and Dr. Basten, an expert witness in population genetics and statistics, testified that the chances of another Black being the source of the semen from Mrs. P’s swabs and panties was 1 in 240 billion.10 Dr. Basten elaborated that if the possibility of population substructures was taken into account,11 that is, the possibility of a slightly higher level of relatedness in certain racial groups or parts of a population, such as Blacks in the United States, then the probability of some unrelated individual other than appellant being the source of the semen changed from 1 in 240 billion to 1 in 2.9 billion.
Trial defense counsel thoroughly cross-examined Mr. Auvdel regarding USACIL DNA [525]*525testing procedures. Mr. Auvdel agreed that the 1992 NRC Report indicated that forensic DNA testing is not infallible, and that “[l]ab-oratory errors happen, even in the best laboratories, and even when the analyst is certain that every precaution against error was taken.” Mr. Auvdel explained that a “match” means only that the observation “falls within [ ] certain criteria, plus or minus 2.5 percent ____” He further explained that DNA analysis does not compare the actual size of DNA bands, but relies on approximations that “fall into a certain range” of plus or minus 2.5 percent. There are six primary steps in DNA analysis, and each primary step is comprised of numerous lesser steps. Risk of cross-contamination requires care to avoid mixing up samples. Mr. Auvdel defended USACIL and FBI use of ethidium bromide, even though the 1992 NRC Report recommended against its use.12
Mr. Auvdel said he knew of the case in England where there was a reported “match” in a DNA test of six chromosome loci, but later testing of additional loci showed the samples did not actually match.13 Many labs, including USACIL, are now going to PCR testing, which tests more loci. Mr. Auvdel agreed with the 1992 NRC Report’s conclusion that blind proficiency testing is superior to open proficiency testing. Mr. Auvdel had conducted “at least two blind proficiency tests.” He could not say, however, whether a blind proficiency test had been made in USACIL’s DNA section the previous year. Mr. Auvdel testified that USACIL did not have a DNA error rate.14
During Mr. Auvdel’s cross-examination the following exchange occurred:
Q. Now, the NRC discusses that perhaps one way of quality assurance would be a second lab test,15 send the samples to a second lab, correct?
A. Yes, sir.
Q. Obviously you don’t do that at USA-CIL?
A. No, sir.
Q. But would you agree that if that was done, that that might increase the confidence in the level of the testing if there were similar results?
A. I believe so, sir, yes.
During Mr. Auvdel’s redirect examination, trial counsel asked whether samples remained of Mrs. P’s vaginal swabs and panties for additional or independent testing. Mr. Auvdel responded, “yes.” The trial counsel then asked whether there had been a request for additional testing by either party. Trial defense counsel objected, arguing that this question was outside the scope of cross-examination and an improper attempt to shift the burden of proof to the defense in violation of appellant’s constitutional rights. Trial counsel countered that the defense had “opened the door” to this question by asking Mr. Auvdel if the 1996 NRC Report, which had made recommendations about lab procedures and quality assurance, was in place in [526]*526the lab when appellant’s DNA samples were tested in 1995. Trial counsel argued, “There’s a clear implication here that had the test been re-done under the new [1996] standards, that there may have been a different result.” The military judge overruled the defense objections and permitted Mr. Auvdel to respond that no one had requested a retest.
Trial defense counsel did not request, nor did the military judge offer to the defense or provide to the members, a limiting instruction. In closing arguments, while trial defense counsel argued that additional testing might exonerate appellant, trial counsel did not mention the possibility of a retest and conceded that the burden of proof rested with the government. At the conclusion of the merits portion of the trial, the military judge instructed the members that the burden of proof remains with the government.
Discussion
In this appeal appellant reasserts a claim made at trial-that when the military judge allowed Mr. Auvdel to testify that no one had requested a DNA retest, the burden of proof effectively shifted from the government to the defense. The government contends that the assignment of error is without merit for three reasons: (1) there was no error; (2) any alleged error was “invited” by trial defense counsel;16 and (3) even if there was error, it was harmless because of the military judge’s subsequent instructions. We agree with the second and third government arguments; we need not address the first.17
The government has the burden of proving that appellant is guilty of each element of the crime beyond a reasonable doubt. UCMJ art. 51(c)(4), 10 U.S.C. § 851(c)(4); United States v. Czekala, 42 M.J. 168, 170 (1995) (citing In re Winship, 397 U.S. 358, 363-64, 90 S.Ct. 1068, 25 L.Ed.2d 368 (1970)). A trial counsel may not comment, directly or otherwise, that an appellant did not testify in his own defense.18 “ ‘But where ... the prosecutor’s reference to the defendant’s opportunity to testify is a fair response to a claim made by defendant or his counsel, we think there is no violation of the privilege.’” Stadler, 47 M.J. at 212 (Crawford, J., concurring in the result) (alteration in original) (quoting United States v. Robinson, 485 U.S. 25, 32, 108 S.Ct. 864, 99 L.Ed.2d 23 (1988)). “ ‘It is well established that the government may comment on the failure of a defendant to refute government evidence or to support his own claims.’”19
While not every comment from a trial counsel about an accused’s failure to testify in his own defense is a constitutional violation, in our analysis we look to the context of the facts in each case,20 and remain vigilant to the fact that appellant had no obligation to request a retest. See UCMJ art. 51(c)(4); Czekala, 42 M.J. at 170. We hold that trial [527]*527defense counsel’s cross-examination of Dr. Auvdel opened the door for trial counsel’s question about DNA retesting by raising the issue of whether further testing of the available DNA material from the rape could have exonerated appellant. We find that trial counsel’s question asking if anyone had requested a retest was a “fair response” to trial defense counsel’s implication. See Gilley, 56 M.J. at 120 (citing Robinson, 485 U.S. at 32, 108 S.Ct. 864) (other citations omitted).
Assuming arguendo, that Mr. Auvdel’s response raised an improper inference relating to appellant’s right not to testify21 or shifted the burden of proof, we find that any error was harmless beyond a reasonable doubt. See Gilley, 56 M.J. at 120; Mobley, 31 M.J. at 279 (both citing Chapman v. California, 386 U.S. 18, 87 S.Ct. 824, 17 L.Ed.2d 705 (1967)). At the close of all evidence the military judge correctly instructed the members as follows: “[T]he burden of proof to establish the guilt of the accused beyond a reasonable doubt is on the government. The burden never shifts to the accused to establish his innocence or to disprove those facts which are necessary to establish each element of any particular offense.” While it would have been better if the military judge had offered the defense counsel an opportunity to request an immediate instruction, such as “ ‘the accused has no duty to produce any evidence in this courtroom ____ you must make sure you do not try to shift the burden to the accused to prove his innocence; that [burden] always rests upon the prosecution[,]’”22 such an offer is not required.23 The instructions to the members immediately before deliberations rendered any error harmless. Stadler, 47 M.J. at 208 (subsequent instructions cured any error arising from improper cross-examination questions concerning appellant’s failure to contact witnesses who could have exonerated him). We presume that the members followed the military judge’s instruction. See United States v. Taylor, 53 M.J. 195, 198 (2000) (citations omitted); United States v. Barron, 52 M.J. 1, 5 (1999) (citing United States v. Rushatz, 31 M.J. 450, 456 (C.M.A.1990)).
Harmless Error and Factual Sufficiency
Absent trial counsel’s question about DNA retesting, it is also clear beyond a reasonable doubt that the panel would have returned a guilty verdict. See United States v. Hasting, 461 U.S. 499, 510-11, 103 S.Ct. 1974, 76 L.Ed.2d 96 (1983) (citation omitted). Moreover, we are independently convinced beyond a reasonable doubt that appellant is guilty. See UCMJ art. 66(c); United States v. Washington, 57 M.J. 394, 399-400 (2002); United States v. Turner, 25 M.J. 324, 325 (C.M.A.1987).
Appellant’s other six primary arguments in support of his claim of factual insufficiency are weak: (1) the timing and circumstances of the rape indicated advance planning, but appellant lacked an opportunity to plan the rape;24 (2) the real rapist seemed to be [528]*528familiar with the P quarters, however, there was no evidence that appellant had such familiarity; 25 (3) appellant’s fertility26 was inconsistent with the lack of motile sperm found during Mrs. P’s rape kit examination shortly after the rape;27 (4) the rape and burglary are inconsistent with appellant’s good military character, as attested to by two noncommissioned officers; (5) appellant’s schedule the morning of the rape made it very difficult for appellant to have committed the crimes;28 and (6) the real rapist removed one of his gloves during the rape,29 a latent fingerprint of unknown origin (not matching appellant) was found on the inside of the doorknob to the front door of the P residence, and this fingerprint might belong to the real rapist.30
Mrs. P described her rapist as a stocky black male, about 5’6” to 5’7" tall, weighing 150 to 160 pounds, with a large nose, and razor bumps; appellant substantially met this description. Appellant had been issued the same type of glove as that left behind by the rapist. Most importantly, the blood type and the DNA from the semen taken from Mrs. P and her panties matched appellant’s blood type and DNA. We are persuaded that appellant’s guilt was proven beyond a reasonable doubt.
All remaining assignments of error are without merit. We find no material prejudice to the substantial rights of appellant. See UCMJ art. 59(a), 10 U.S.C. § 859(a).
The findings of guilty and the sentence are affirmed
Senior Judge CANNER and Judge BARTO concur.